{"id":6443,"date":"2026-03-10T08:05:37","date_gmt":"2026-03-10T01:05:37","guid":{"rendered":"https:\/\/ogrindoitb.com\/?p=6443"},"modified":"2026-05-24T06:45:51","modified_gmt":"2026-05-23T23:45:51","slug":"training-hplc-rid-part-1-pengenalan-sistem-untuk-analisis-yang-presisi","status":"publish","type":"post","link":"https:\/\/ogrindoitb.com\/en\/training-hplc-rid-part-1-pengenalan-sistem-untuk-analisis-yang-presisi\/","title":{"rendered":"Training HPLC \u2013 RID Part 1: System Introduction for Precise Analysis"},"content":{"rendered":"

In an effort to maintain accurate, precise, and reproducible<\/em>analytical quality standards, OGRINDO ITB and the EOR Laboratory ITB organized an HPLC\u2013RID Training in collaboration with PT. Berca Niaga Medika. This activity aims to strengthen the team's understanding of the High Performance Liquid Chromatography<\/em> (HPLC) system with a Refractive Index Detector<\/em> (RID), ensuring optimal instrument operation and generating reliable data to support research and applications of Chemical<\/em> EOR.<\/p>\n\n\n

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Figure 1. Technical discussion session during the HPLC\u2013RID Training with the team from PT. Berca Niaga Medika at the EOR Laboratory ITB.<\/figcaption><\/figure>\n<\/div>\n\n
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Figure 2. Training participants enthusiastically taking part in the discussion and introduction session of the HPLC\u2013RID system at the EOR Laboratory ITB.<\/figcaption><\/figure>\n<\/div>\n\n\n
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HPLC 1260 \u2013 RID System at the EOR Laboratory ITB<\/strong><\/p>\n\n\n\n

The EOR Laboratory ITB utilizes an HPLC type 1260 equipped with an RID detector system and a manual injector<\/em>. This configuration is highly suitable for analyzing compounds such as polymers and surfactants, particularly in studies of chemical<\/em> adsorption onto rock, detection and quantification of polymers and surfactants in monitoring wells, as well as evaluation of injection performance in Chemical<\/em> EOR schemes. Understanding each component is key to maintaining system stability and ensuring the quality of analytical results.<\/p>\n\n\n

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Figure 3. The instructor explaining the configuration and main components of the HPLC\u2013RID system used in the sample analysis process.<\/figcaption><\/figure>\n<\/div>\n\n\n
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Main Components and Their Functions<\/strong><\/p>\n\n\n\n

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  1. Mobile Phase Reservoir
    A container used to store the solvent (mobile phase<\/em>) that will flow through the system. The quality and cleanliness of the mobile phase greatly determine pressure stability and the baseline<\/em> chromatogram.<\/li>\n\n\n\n
  2. Isocratic Pump
    The system used is isocratic, meaning it uses a single, constant mobile phase composition throughout the analysis. In contrast, gradient<\/em> systems allow changes in the composition of 2\u20134 solvents through software<\/em>control, isocratic systems are simpler and more stable for routine methods with relatively consistent sample matrices.<\/li>\n\n\n\n
  3. Manual Injector
    The injection process is carried out manually using a 20 \u00b5L loop and a precision syringe<\/em> (typically 50 \u00b5L) to ensure consistent injection volume and repeatability<\/em> maintain.<\/li>\n\n\n\n
  4. HPLC Column
    The column is the core of the separation process. The column compartment is equipped with a heater <\/em>with temperatures up to 85\u00b0C to maintain temperature stability and consistency retention time<\/em>.<\/li>\n\n\n\n
  5. Refractive Index Detector<\/em> (RID)
    RID operates based on differences in refractive index between the mobile phase and sample components. This detector is highly sensitive to temperature changes, solvent composition, and the presence of air bubbles, making system stability a crucial factor.<\/li>\n<\/ol>\n\n\n
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    Figure 4. Demonstration of the sample injection process using a syringe<\/em> in the system of manual injector<\/em> HPLC.<\/figcaption><\/figure>\n<\/div>\n\n
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    Figure 5. Internal view of the HPLC system showing the flow path of the mobile phase toward the column and detector.<\/figcaption><\/figure>\n<\/div>\n\n\n
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    System Stability Begins with the Mobile Phase<\/strong><\/p>\n\n\n\n

    One of the main topics discussed during the training<\/em> was the importance of ensuring that the mobile phase is free from air bubbles (bubble<\/em>).<\/p>\n\n\n\n

    Indications that the System Contains Bubble<\/em>:<\/p>\n\n\n\n